一例IgM+IgG型抗-K抗体鉴定与分析OA
A Case of Mixed IgM and IgG Anti-K Antibody:Identification and Clinical Implications
目的 对一例不规则抗体筛查阳性患者血清中发现的抗-K抗体进行鉴定,测定抗体的性质及效价,并分析产生的原因.方法 采用血清学方法检测患者的ABO血型及Rh分型;采用盐水介质试管法(NS)、微柱凝胶法(MGT)进行不规则抗体筛查及鉴定;交叉配血采用NS、MGT及手工凝聚胺法(MPT)进行;采用2-巯基乙醇(2-Me)试剂处理患者血清测定抗体的性质及效价.结果 患者血清不规则抗体筛查为阳性,特异性鉴定为抗-K抗体,性质为IgM+IgG型,其中IgM型效价为2,IgG型效价为256.结论 利用合适的实验室检测方法可以准确鉴定出血清中存在的IgM+IgG型抗-K抗体,为临床输血提供安全保障.
Objective To identify the specificity,immunoglobulin subclass and titer of an anti-K antibody detected in a patient with a positive irregular antibody screen,and to analyze potential etiological factors contributing to its formation.Methods ABO blood grouping and Rh phenotyping were performed using standard serological methods.Irregular antibody screening and identification were carried out by normal saline(NS)tube test and microcolumn gel test(MGT).Crossmatching was conducted using NS,MGT,and manual polybrene test(MPT).The serum was treated with 2-mercaptoethanol(2-ME)to determine the immunoglobulin class and titer of the antibody.Results The irregular antibody screen was positive,and the antibody was specifically identified as anti-K.It was characterized as a mixed IgM and IgG isotypes,with an IgM titer of 2 and an IgG titer of 256.Conclusion Utilization of suitable laboratory techniques allows for the accurate detection of IgM+IgG anti-K antibodies,providing a guarantee for safe clinical transfusion.
康世蓉;王丹;袁小华;陆红芹;李苏亮
西安医学院第一附属医院,陕西 西安 710000西安医学院第一附属医院,陕西 西安 710000西安医学院第一附属医院,陕西 西安 710000西安医学院第一附属医院,陕西 西安 710000西安医学院第一附属医院,陕西 西安 710000
医药卫生
Kell血型抗-K抗体抗体鉴定IgM抗体IgG抗体
Kell blood groupAnti-K antibodyAntibody identificationIgM antibodyIgG antibody
《临床输血与检验》 2026 (3)
425-429,5
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