不同检测方法与不同酶处理的红细胞对抗-E、抗-c的敏感度分析OA

Objective This study aimed to investigate the effects of treating red blood cells(RBCs)with four different proteinaases on the detection of anti-E and anti-c antibodies,and to compare the sensitivity among three detection methods.The goal is to provide a scientific basis for selecting the optimal enzyme treatment detection protocol in clinical practice.Methods Five RBC samples with different Rh phenotypes(3 cases of CcEe,1 case of Ccee,and 1 case of CCEe)were selected.RBCs were treated using a two-step enzyme method with chymotrypsin,trypsin,pronase,and papain.Prior to enzyme treatment,antibody titers for anti-E and anti-c were determined by the Microcolumn Gel Test(MGT),the polybreneethylene Glycol-enhanced Microcolumn Gel Test(PEG-MGT),and the Polybrene method.Following enzyme treatment,the MGT method was used uniformly for antibody detection.Cells with CcEe and CCEe phenotypes were tested for anti-E;cells with CcEe and Ccee phenotypes were tested for anti-c.Analyses were performed on:differences among the three pre-treatment methods;differences among the four enzyme treatment groups;and different titers between post-enzyme treatment and pre-enzyme treatment.Results Anti-E results:Anti-E:Before enzyme treatment,a significant difference in detected titers was found between the PEG-MGT and MGT method(P＜0.05).After treatment with the four enzymes,a significant difference in titer was observed between chymotrypsin and pronase(P＜0.05).When comparing the titers of MGT after enzyme treatment with those before treatment,significant differences were observed between the pronase and MGT groups,as well as between the papain and MGT groups(P＜0.05).Anti-c results:Before enzyme treatment,a significant difference in detected titers was found between the Polybrene and MGT methods(P＜0.05).After treatment with the four enzymes,a significant difference in titer was observed between chymotrypsin and papain(P＜0.05).When comparing post-enzyme treatment MGT titers with pre-enzyme treatment titers,significant differences were found between the MGT method and the trypsin,pronase,and papain groups(P＜0.05).Conclusion Before enzyme treatment,the optimal method for detecting anti-E is PEG-MGT,while the optimal method for detecting anti-c is the Polybrene method.After enzyme treatment,the pronase-MGT combination is the best solution for detecting anti-E,whereas the papain-MGT combination serves as the optimal method for detecting anti-c.Enzyme treatment techniques and PEG can significantly enhance the detection sensitivity of specific antibodies,serving as an important supplement to conventional detection methods.