冻干富血小板血浆对体外人真皮成纤维细胞增殖和迁移的影响OA
Effect of Freeze-dried Platelet-rich Plasma on the Proliferation and Migration of Human Dermal Fibroblasts in Vitro
目的 研究冻干富血小板血浆(freeze-dried platelet-rich plasma,FD-PRP)对人真皮成纤维细胞(human dermal fibroblasts,HDFs)增殖和迁移的作用.方法 分离制备PRP并进行冷冻干燥处理得到FD-PRP,CCK-8法检测人成纤维细胞增殖情况,细胞划痕实验检测人真皮成纤维细胞迁移情况,ELISA检测Ⅰ型胶原蛋白、弹性蛋白的含量.结果 与空白对照组相比,FD-PRP能够显著增加HDFs的增殖以及Ⅰ型胶原蛋白和弹性蛋白表达水平(P＜0.05),而与PRP组相比差异无统计学意义(P＞0.05).此外,细胞划痕实验表明,相较于空白组对照组,FD-PRP和PRP处理后的细胞明显向"划痕"处迁移.结论 综上所述,FD-PRP诱导成人真皮纤维细胞增殖和迁移,同时能够刺激Ⅰ型胶原、弹性蛋白的分泌增加.这一研究为FD-PRP日后用于临床治疗各类创面奠定了研究基础.
Objective To evaluate the capacity of freeze-dried platelet-rich plasma(FD-PRP)to stimulate the proliferation and migration of human dermal fibroblasts.Methods Freshly isolated PRP was lyophilized to generate FD-PRP.Cell proliferation was quantified using the CCK-8 assay;directional migration was monitored via scratch-wound assays;and the secretion of type Ⅰ collagen and elastin was measured by ELISA.Results Compared to untreated controls,FD-PRP significantly enhanced fibroblast proliferation and increased the secretion of type Ⅰ collagen and elastin(P＜0.05).These effects were statistically comparable to those induced by fresh PRP(P＞0.05).Scratch-wound assays further revealed that both FD-PRP and fresh PRP markedly enhanced cell migration into the denuded area compared with the controls.Conclusion FD-PRP induces the proliferation and migration of adult dermal fibroblasts and also enhances the secretion of type Ⅰ collagen and elastin.These findings lay a solid experimental foundation for the potential clinical application of FD-PRP in the future treatment of various wound types.
李艳辉;皮丽萍;谢婷婷;周谋;施琳颖
中国人民解放军南部战区总医院,广东 广州 510010中国人民解放军南部战区总医院,广东 广州 510010中国人民解放军南部战区总医院,广东 广州 510010中国人民解放军南部战区总医院,广东 广州 510010中国人民解放军南部战区总医院,广东 广州 510010
医药卫生
富血小板血浆冻干人成纤维细胞创面
Platelet rich plasmaFreeze-driedHuman fibroblastsWound
《临床输血与检验》 2026 (3)
359-363,5
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