纤维素酶辅助提取皂荚皂苷的工艺优化及抗氧化活性研究OA

By optimizing the extraction process of saponins from Gleditsia sinensis,a method was developed to improve the yield of saponin extraction and futher explored its antioxidant capacity.Using cellulase assisted extraction of G.sinensis saponins,the extraction yield of saponins was used as the evaluation index.Based on single factor experiments,the extraction process conditions were optimized by response surface methodology,and the scavenging ability of saponins on DPPH and ABTS free radicals was determined to explore their antioxidant capacity.The results showed that the optimal process conditions for extracting saponins from G.sinensis pods were 3％enzyme dosage(based on the mass of pod powder),enzymatic hydrolysis temperature of 50 ℃,enzymatic hydrolysis time of 2.5 h,and pH value of 5.0.Under these conditions,the extraction yield of saponins from G.sinensis was 26.457％,which was 15.451 percentage points higher than the extraction yield without cellulase(11.01％).The concentration of saponins in G.sinensis pods is within the range of 0.1-0.5 g/L,which has a good scavenging effect on DPPH free radicals.The concentration of saponins in the range of 0.1-0.7 g/L has a good scavenging effect on ABTS free radicals,and the scavenging rate increases with the increase of saponin concentration.The scavenging rate of 0.5 g/L saponin solution on DPPH free radicals is 80.81％,significantly lower than vitamin C(Vc),while the scavenging rate of 0.7 g/L saponin solution on ABTS free radicals is 96.63％,which is close to Vc.The extraction yield of saponins from G.sinensis assisted by cellulase is high,and the enzymatic hydrolysis method is more environmentally friendly.The saponins from G.sinensis obtained under the optimal process conditions have good antioxidant properties,and this method is suitable for industrial production.