竹粉中对香豆酸的微波提取工艺优化及其除草活性OA

Bamboo powder derived from bamboo processing residues was extracted using a microwave-assisted deep eutectic solvent(DES,choline chloride-lactic acid)system to explore its application potential in the field of botanical herbicides.p-Coumaric acid,ferulic acid and other components were identified in the extract by liquid chromatography-quadrupole time-of-flight mass spectrometry.With the yield of p-coumaric acid as the index,the microwave-assisted extraction process of bamboo powder was optimized by combining single-factor and orthogonal experiments.The optimal extraction process conditions were obtained as follows:1g bamboo powder,liquid-solid ratio of choline chloride-lactic acid to bamboo powder of 6.9∶1(mL∶g),extraction time of 3.5 min and microwave power of 420 W.Under these conditions,the yield of p-coumaric acid reached 3.20 mg/g.The herbicidal activity of the bamboo powder extract was evaluated using the water-agar medium method,and it exhibited inhibitory effects on the root length,shoot length,and fresh weight of the target weed Trifolium pratense L.,showing a clear dose-response relationship.Among the three parameters,root length was the most sensitive to the active components.The herbicidal toxicity of the extract against root length(IC50=152.95 mg/L)was significantly weaker than that of p-coumaric acid(IC50=63.25 mg/L).This confirms that p-coumaric acid is the main herbicidal active component in the bamboo powder extract.