搭载依达拉奉的活性氧响应型水凝胶的构建及其对成骨细胞的影响和抑菌性的研究OA

Objective:To construct an Edaravone-loaded reactive oxygen species(ROS)-responsive hydrogel and to evaluate its physicochemical properties,osteogenic effects and antibacterial properties.Methods:The composite hydrogel was prepared using the freeze-thaw cycle method.The working concentration of Edaravone was screened using the CCK-8 assay.The composition ratios were then optimized through porosity,mechanical property,swelling rate and degradation rate tests to identify the optimal composite hydrogel,and the drug release behavior and ROS scavenging ability were evaluated.The hydrogel was co-cultured with Staphylococcus aureus,and its antibacterial activity was assessed by the colony counting method.After co-culturing the hydrogel with mouse embryo osteoblast precursor cells(MC3T3-E1 cells),alkaline phosphatase(ALP)staining,alizarin red S(ARS)staining and real-time quantitative polymerase chain reaction(RT-qPCR)were used to evaluate the effects of the hydrogel on the proliferation,mineralization and osteogenesis-related gene expression of MC3T3-E1 cells.Results:A composite hydrogel(P3ATE)with a three-dimensional porous network structure was successfully prepared.It exhibited favorable mechanical,swelling and degradation properties,and provided sustained release of Edaravone.It demonstrated strong ROS scavenging ability(P<0.05)and good antibacterial activity,significantly inhibiting the growth of Staphylococcus aureus(P<0.05).Compared with the control group,the P3ATE hydrogel significantly promoted the proliferation and mineralization of MC3T3-E1 cells,enhanced ALP activity,and upregulated the expression of Bmp2,Spp1,Runx2 and Col1a1(P<0.05).Conclusion:The constructed Edaravone-loaded ROS-responsive composite hydrogel exhibits favorable osteogenic,antioxidant and antibacterial properties,suggesting potential application value in the repair of jaw defects.