尿液BTA与NMP22联合检测在膀胱癌诊断中的应用OA
Combined detection of urinary BTA and NMP22 in the diagnosis of bladder cancer
目的 评估基于化学发光免疫分析法的尿液膀胱肿瘤抗原(BTA)和核基质蛋白22(NMP22)单项及联合检测在膀胱癌诊断中的价值,并分析其与膀胱癌病理分级的关系.方法 选取2022年8月至2024年12月该院收治的膀胱癌患者80例(膀胱癌组)、泌尿系统其他恶性肿瘤患者51例(泌尿系统其他恶性肿瘤组)、泌尿系统良性疾病66例患者(泌尿系统良性疾病组)以及同期在该院体检的健康体检者50例(健康对照组)作为研究对象.根据肿瘤肌层浸润状态分为肌层浸润性膀胱癌(MIBC)和非肌层浸润性膀胱癌(NMIBC);按病理分级分为G1、G2、G3级.采用全自动化学发光免疫分析平台检测所有研究对象尿液BTA 和 NMP22水平.通过受试者工作特征(ROC)曲线评估尿液BTA、NMP22对膀胱癌的诊断效能.通过Spearman相关分析膀胱癌患者尿液BTA、NMP22水平与膀胱癌病理分级的相关性.结果 与泌尿系统其他恶性肿瘤组、泌尿系统良性疾病组及健康对照组相比,膀胱癌组尿液 BTA 及 NMP22水平显著升高,差异均有统计学意义(P<0.008 3).ROC曲线分析显示,尿液 BTA、NMP22单项及联合诊断膀胱癌的曲线下面积(AUC)分别为0.748、0.768、0.780.MIBC患者 BTA 和 NMP22水平显著高于 NMIBC患者,差异均有统计学意义(P<0.05).不同病理分级患者尿液BTA及NMP22水平比较,差异均有统计学意义(P<0.05).与 G1、G2级患者比较,G3级患者尿液BTA和NMP22水平显著升高,差异均有统计学意义(P<0.016 7).Spearman相关分析结果显示,膀胱癌患者尿液 BTA、NMP22水平与膀胱癌的病理分级呈正相关(rs=0.295、0.301,均P<0.01).结论 尿液BTA与 NMP22对膀胱癌均具有一定的诊断价值,二者联合检测在整体上可提高诊断效能,二者均可作为膀胱癌辅助诊断的参考指标.
Objective To evaluate the diagnostic value of urinary bladder tumor antigen(BTA)and nuclear matrix protein 22(NMP22)detected by chemiluminescence immunoassay,both alone and in combination,for bladder cancer,and to analyze their correlations with pathological grade.Methods A total of 247 subjects who visited the hospital or underwent physical examination from August 2022 to December 2024 were selected,comprising 80 patients with bladder cancer(bladder cancer group),51 with other urological malignancies(other urological malignancies group),66 with benign urological diseases(benign urological diseases group),and 50 healthy individuals(healthy control group).Based on muscular invasion status,patients with bladder cancer were divided into muscle-invasive bladder cancer(MIBC)and non-muscle-invasive bladder cancer(NMIBC);based on pathological grade,they were divided into G1,G2,and G3 grades.The levels of urinary BTA and NMP22 in all subjects were measured using a fully automated chemiluminescence immunoassay plat-form.The diagnostic performance of urinary BTA and NMP22 for bladder cancer was evaluated using receiver operating characteristic(ROC)curve.Correlations of urinary BTA and NMP22 levels with the pathological grade of bladder cancer were analyzed using Spearman correlation analysis.Results Compared with the other uro-logical malignancies group,the benign urological diseases group and the healthy control group,urinary BTA and NMP22 levels were elevated in the bladder cancer group,and the differences were statistically significant(all P<0.008 3).The areas under the ROC curves(AUCs)of urinary BTA alone,NMP22 alone,and their combination for the diagnosis of bladder cancer were determined to be 0.748,0.768,and 0.780 respectively by ROC curve analysis.Regarding pathological features,significantly elevated BTA and NMP22 levels were measured in the MIBC patients compared with the NMIBC patients(P<0.05).Statistically significant differ-ences in these marker levels were found among patients with different pathological grades(P<0.05);specif-ically,compared with patients with G1 and G2 tumors,significantly higher urinary BTA and NMP22 levels were recorded in patients with G3 tumors(P<0.016 7).Positive correlations of urinary BTA and NMP22 levels with the pathological grade of bladder cancer were demonstrated by Spearman correlation analysis(rs=0.295 and 0.301 respectively;both P<0.01).Conclusion Both urinary BTA and NMP22 have certain diag-nostic value for bladder cancer,and their combined detection improves the overall diagnostic performance.These two markers can serve as reference indicators for the auxiliary diagnosis of bladder cancer.
续晴云;黄海明;李海霞
北京大学第一医院检验科,北京 100034北京大学第一医院检验科,北京 100034北京大学第一医院检验科,北京 100034
医药卫生
膀胱癌膀胱肿瘤抗原核基质蛋白22化学发光免疫分析法诊断
bladder cancerbladder tumor antigennuclear matrix protein 22chemiluminescence immunoassaydiagnosis
《检验医学与临床》 2026 (12)
1605-1610,6
北京高层次创新创业青年拔尖人才青苗计划项目(G202522094).
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