甘草酸二钾抑制组胺激活细胞以缓解气道高反应性OA

The study aims to establish a histamine-induced Airway Smooth Muscle Cells(ASMC)in-flammation model.Through cell viability assays,gelatin zymography,flow cytometry analysis,west-ern blot analysis,and immunofluorescence staining,the effects of Dipotassium Glycyrrhizinate(DG)on airway smooth muscle cells were investigated,and the potential pharmacological mechanism of DG in alleviating airway hyperresponsiveness was explored.The results showed that DG inhibited hista-mine-induced cell proliferation and migration by suppressing the Protease-Activated Receptor-2(PAR-2)/Toll-Like Receptor-4(TLR-4)/Nuclear Factor-kappa B(NF-κB)signaling pathway and the inte-grin-β1/Focal Adhesion Kinase(FAK)signaling pathway.DG regulated the cell cycle and induced ap-optosis of overactivated ASMC by upregulating the expression of tumor suppressor genes(p53),Cys-teine aspartate protease 3(Caspase3),and autophagy effector protein(Beclin1),and downregulating the expression of Proliferating Cell Nuclear Antigen(PCNA).Through molecular docking simula-tions,DG demonstrated high affinity for binding to histamine receptors,PAR-2 receptors,and TLR-4 receptors.The study indicates that DG can inhibit histamine-induced overactivation and proliferation of ASMC,and reveals the potential for multi-target regulation,unveiling DG's antihistamine function and its potential pharmacological mechanism in alleviating airway hyperresponsiveness.