硫酸葡聚糖抑制ALV-J复制及其机制初探OA

In order to evaluate the inhibitory effect of dextran sulfate(DS)on the replication of avian leukosis virus subgroup J(ALV-J)and its potential mechanism,a systematic study was conducted by combining in vitro and in vivo ex-periments.The safety of low(0.2 g·kg-1),medium(1 g·kg-1)and high(5 g·kg-1)doses of DS was evaluated by gavage to healthy chicks,and the effects on the growth status and weight of chicks were observed.Subsequently,a chick-en model infected with ALV-J was established,and the same dose of DS was used to intervene.The viral load in immune organs such as liver,spleen and bursa of Fabricius was detected by qPCR.In vitro,MTT and CCK-8 methods were used to evaluate the cytotoxicity of DS to chicken macrophage cell line HD11,qPCR and Western blot were used to detect the effects of DS on the gene transcription and protein expression of virus envelope protein(env)after ALV-J infected HD11 cells,and the expression level of inflammatory factor IL-1β was also analyzed.The results showed that DS had no obvious cytotoxicity to HD11 cells in the dose range of 0.2-5 g·kg-1,and the chicks had no obvious toxic and side effects after gavage,and their growth status was normal.DS intervention can significantly reduce the viral load in immune organs of chickens infected with ALV-J,and significantly reduce the mRNA and protein expression level of ALV-J env gene in HD11 cells.In addition,DS can up-regulate the expression of IL-1β in a concentration and time-dependent manner.To sum up,DS can effectively inhibit the replication of ALV-J by activating the host inflammatory response(up-regulating IL-1β),and it has good safety,which provides a theoretical basis for the development of new immunomodulators for the prevention and treatment of avian leukemia.