盐碱植物根际溶磷菌的筛选及对玉米生长的影响OA
Screening of phosphorus-solubilizing bacteria in the rhizosphere of saline-alkali plants and their effects on maize growth
为进一步挖掘耐盐促生菌种资源,研制适用于盐碱环境的植物促生菌剂,本研究利用选择性培养基,从新疆维吾尔自治区喀什地区盐碱草地植物根际土壤中分离筛选溶磷菌,通过16S rRNA基因测序鉴定菌株分类地位,并采用半固体试管苗和盐碱土盆栽试验评估其对植物的促生效应.结果共筛选出19株溶磷菌菌株,溶磷量介于7.67～43.08 μg/mL,菌株c3-9、f1-8、b1-8、c3-1溶磷量位列前4位,且具有一定耐盐碱胁迫能力.19株溶磷菌菌株隶属于6个菌属,包括肠杆菌属(Enterobacter)、不动杆菌属(Acinetobacter)、假单胞菌属(Pseudomonas)、芽孢杆菌属(Bacillus)、金色单胞菌属(Chryseomonas)、勒克氏菌属(Leclercia).接种假单胞菌属f1-8菌株、不动杆菌属b1-8菌株、霍氏肠杆菌属c3-1菌株和勒克氏菌属c3-9菌株对非盐碱胁迫下玉米试管苗生长有不同程度的促进作用.盐碱土壤盆栽试验结果显示,与对照相比,f1-8菌株显著提升了玉米株高和地上部鲜重(P＜0.05),c3-9菌株对玉米根干重有显著促进作用(P＜0.05),f1-8菌株、c3-1菌株对玉米总根体积的影响达显著水平(P＜0.05),f1-8菌株、b1-8菌株、c3-9菌株对玉米总根长的影响达显著水平(P＜0.05).此外,盆栽土壤理化性质检测结果显示,接种溶磷菌后土壤速效磷含量增加了 16.03％～66.79％.对玉米生长指标和土壤速效磷含量的主成分分析结果表明,c3-9处理的综合效果最佳.该研究结果扩充了盐碱地溶磷菌菌种资源库,为开发盐碱地改良微生物肥料提供了理论基础.
To further explore salt-tolerant and growth-promoting microbial resources and develop plant growth-promoting bacterial inoculants suitable for saline-alkali environments,this study employed selective media to isolate and screen phosphate-solubilizing bacteria from the rhizosphere soil of plants growing in saline-alkali grasslands in Kashi,Xinjiang.The taxonomic status of the strains was identified through 16S rRNA gene sequencing,and their growth-promoting effects on plants were evaluated using semi-solid tube plantlets and pot experiments with saline-alkali soil.A total of 19 phosphate-solubilizing bacterial strains were screened,with the amounts of solubilized phosphorus ranging from 7.67 μg/mL to 43.08 μg/mL.The strains c3-9,f1-8,b1-8 and c3-1 ranked among the top four in terms of phosphate-solubilizing amounts and exhibited a certain tolerance to saline-alkali stress.The 19 phosphate-solubilizing bacterial strains belonged to six genera,including Enterobacter,Acinetobacter,Pseudomonas,Bacillus,Chryseomonas,and Leclercia.Inoculation with Pseudomonas strain f1-8,Acinetobacter strain b1-8,Enterobacter hormaechei strain c3-1,and Leclercia strain c3-9 all promoted the growth of maize plantlets under non-saline-alkali stress to varying degrees.Pot experiments with saline-alkali soil showed that,compared with the control,strain f1-8 significantly increased maize plant height and above-ground fresh weight(P＜0.05);strain c3-9 significantly promoted maize root dry weight(P＜0.05);strains f1-8 and c3-1 significantly affected total root volume(P＜0.05);and strains f1-8,b1-8,and c3-9 significantly influenced total root length(P＜0.05).Additionally,the physicochemical property analysis of the potting soil indicated that the available phosphorus content increased by 16.03％to 66.79％after inoculation with phosphate-solubilizing bacterial strains.The principal component analysis(PCA)results for maize growth indices and soil available phosphorus content indicated that the c3-9 treatment exhibited the best overall effect.These findings expand the germplasm resource library of phosphate-solubilizing bacteria for saline-alkali soils and provide a theoretical basis for the development of microbial fertilizers for saline-alkali soil improvement.
古鸣;李明源;李云霞;王继莲
喀什大学生命与地理科学学院/新疆帕米尔高原生物资源与生态重点实验室,新疆喀什 844000喀什大学生命与地理科学学院/新疆帕米尔高原生物资源与生态重点实验室,新疆喀什 844000喀什大学生命与地理科学学院/新疆帕米尔高原生物资源与生态重点实验室,新疆喀什 844000喀什大学生命与地理科学学院/新疆帕米尔高原生物资源与生态重点实验室,新疆喀什 844000
农业科技
盐碱地溶磷菌促进生长玉米
saline-alkali soilphosphate-solubilizing bacteriagrowth promotionmaize
《江苏农业学报》 2026 (5)
963-972,10
喀什地区科技计划项目(KS2024001)国家自然科学基金项目(32160408)
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