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小麦籽粒大小相关稳定QTL的初步定位OA

Preliminary mapping of the stable QTLs associated with wheat grain size

中文摘要英文摘要

为了挖掘更多调控小麦籽粒大小的稳定数量性状位点(QTL),本研究以扬麦158与西风杂交构建的重组自交系群体为材料,结合连续4年不同环境下的籽粒面积、籽粒长度、籽粒宽度及籽粒长宽比表型数据以及群体55K单核苷酸多态性(SNP)芯片基因型信息,开展与小麦籽粒大小相关的稳定QTL定位分析.结果分别定位到3个与籽粒面积显著相关、7个与籽粒长度显著相关、4个与籽粒宽度显著相关、2个与籽粒长宽比显著相关的稳定QTL,分布于小麦2A、2D、3A、3D、4A、4B、4D、5A、5B和5D染色体上,单个稳定QTL可解释3.60%~19.60%的表型变异.其中的 QGA.jaas-3A、QGA.jaas-4B、QGA.jaas-4D、QGL.jaas-3D、QGL.jaas-4D、QGL.jaas-5B、QGW.jaas-2A、QGW.jaas-4B、QGW.jaas-4D和QLWR.jaas-5D为新发现的QTL.这些与小麦籽粒大小相关稳定QTL可为长江中下游麦区高产小麦的标记辅助选择或分子设计育种提供帮助.

To explore more stable quantitative trait loci(QTLs)associated with wheat grain size,this study used the recombinant inbred line population derived from the cross between Yangmai 158 and Xifeng as the material.By integrating four consecutive years of multi-environment phenotypic data on grain area,grain length,grain width,and grain length-to-width ratio,together with genotypic information from a 55K single nucleotide polymorphism(SNP)chip,a mapping analy-sis was performed to identify stable QTLs associated with wheat grain size.A total of 16 stable QTLs were identified,including three,seven,four,and two QTLs significantly associated with grain area,grain length,grain width,and grain length-to-width ratio,respectively.These QTLs were distributed on the 2A,2D,3A,3D,4A,4B,4D,5A,5B and 5D chromosomes of wheat.A single QTL can explain 3.60%to 19.60%of phenotypic variations.Among them,QGA.jaas-3A,QGA.jaas-4B,QGA.jaas-4D,QGL.jaas-3D,QGL.jaas-4D,QGL.jaas-5B,QGW.jaas-2A,QGW.jaas-4B,QGW.jaas-4D,and QLWR.jaas-5D were newly discovered QTLs.These stable QTLs related to wheat grain size can provide assistance for marker-assisted selection or molecular design breeding of high-yield wheat in the middle and lower reaches of the Yangtze River wheat region.

周淼平;张鹏;杨学明;张平平;宋桂成;何漪

江苏省农业科学院粮食作物研究所,江苏南京 210014江苏省农业科学院粮食作物研究所,江苏南京 210014江苏省农业科学院粮食作物研究所,江苏南京 210014江苏省农业科学院粮食作物研究所,江苏南京 210014江苏省农业科学院粮食作物研究所,江苏南京 210014江苏省农业科学院粮食作物研究所,江苏南京 210014

农业科技

小麦籽粒大小数量性状位点(QTL)重组自交系

wheatgrain sizequantitative trait loci(QTL)recombinant inbred lines

《江苏农业学报》 2026 (5)

900-908,9

国家小麦产业体系基金项目(CARS-03-57)江苏省揭榜挂帅项目(JBGS049)

10.3969/j.issn.1000-4440.2026.05.004

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