低温处理对不同栽培区蓝莓枝条生理特性与花芽形态的影响OA

In order to explore the differences of dormancy characteristics of blueberry varieties in dif-ferent cultivation areas and optimize the regional cultivation and management technology,seven blue-berry varieties in four cultivation areas of Jilin,Shandong,Hubei and Yunnan were used as test mate-rials in this study.The effects of low-temperature treatment combined with artificial culture on the germination process of flower buds were dynamically observed,and the morphological characteristics and tissue differentiation process of flower buds were observed by paraffin section technique.The re-sults showed that the soluble sugar content in the branches showed a gradual upward trend,the starch content decreased as a whole,the soluble protein showed a first decreasing and then increas-ing trend,and the free amino acid content showed a first increasing and then decreasing trend.The activity of antioxidant enzymes reached the peak at the middle stage of dormancy release,and the content of malondialdehyde increased slowly first and then decreased gradually.The results of dy-namic changes of horizontal and vertical diameters of blueberry under artificial culture conditions showed that the horizontal and vertical diameters of flower buds of blueberry varieties in different cultivation areas showed a significant growth trend under artificial culture conditions,but there were significant differences among varieties and regions.The morphological changes of blueberry variet-ies at the early and late stages of flower bud differentiation were observed by paraffin section.The re-sults showed that the internal structure of flower buds was close,the cell division activity was weak,and the primordium of flower organs was not obvious at the early stage of differentiation.At the later stage of differentiation,the primordium structure of calyx,petal,stigma and stamen could be clearly observed,and the cell division activity reached the peak.The time and degree of floral organ differ-entiation of different varieties were different,which was related to the duration of low temperature treatment and light culture.The results of this experiment can provide theoretical basis and techni-cal reference for the selection of blueberry varieties,the optimization of precise temperature control cultivation techniques and the evaluation of cold resistance of new varieties in different climatic re-gions.