辽宁地区圈养与野生东北林蛙肠道细菌多样性比较OA

To investigate the differences in the structure and function of gut bacterial communities between captive and wild Rana dybowskii,12 wild Rana dybowskii and 12 captive Rana dybowskii were collected from Liaoning Province.Samples were taken separately from the duodenum,ileum,and rectum.The microbial community structure was identified using high-throughput sequencing technology for 16S rDNA.Alpha diversity analysis,beta diversity analysis,and cluster analysis were used to compare the differences in microbial communities between the two groups.The results showed that the dominant phyla in the intestinal tract of wild Rana dybowskii were Proteobacteria,Firmicutes,and Actinobacteria,while the dominant genera were Mycobacterium,Pseudomonas,and Bacteroides.The dominant phyla in the captive group were Proteobacteria,Firmicutes,and Bacteroidetes,while the dominant genera were Pseudomonas,Citrobacter,and Shewanella.In the entire gut of Rana dybowskii,the phyla Proteobacteria,Actinobacteria,and Bacteroidetes were the dominant phyla,the relative abundance of Proteobacteria in the wild group was lower than that in the captive group.There was a significant difference in the overall gut bacterial alpha diversity between wild and captive groups of Rana dybowskii(P<0.05),while the beta diversity difference was small.Comparing the bacterial diversity of different intestinal parts between wild and captive Rana dybowskii,the alpha diversity of bacteria in the small intestine and the beta diversity in the rectum differed obviously.The research results provide a scientific basis for investigating the effects of different environments on the intestinal health status and gut function of the Rana dybowskii.