4个影响中蜂囊状幼虫病毒复制的关联因子OA

Chinese sacbrood virus(CSBV)is the main pathogen causing the viral disease of honeybees(Apis cerana cerana).The prevalence of bee viral diseases is correlated with the virus load of honeybee colony.To reveal the causes of the prevalence and outbreak of bee viral diseases,the dynamic changes of CSBV copy in the worker bee samples of the experimental bee colonies before and after treatment with different biological factors(colony population,number of parasitic Varroa destructor,mixed feeding by Eastern and Western honeybees)and abiotic factors(different emergence temperatures)were detected.The experimental data showed that strong colony had significant resistance to CSBV than weak colony.The number of parasitic mites was positively correlated with the CSBV titer of bee samples.The nucleic acid copy number of CSBV in worker bees kept in Western honeybee colonies significantly increased.Emergence temperature fluctuation not only significantly increased the CSBV copy in worker bees,but also sharply reduced the emergence rate of worker bees.The experimental proved that beekeeper of Chinese honey bee could prevent CSBV in maintaining strong colony population,reducing the parasitism rate of varroa mite and dividing with Western honeybee colonies.Optimal emergence temperature of bee also improves colony and prevents CSBV effectively.