基于响应面分析法优化丁酸梭菌HADIG-CB004发酵条件OA

To develop a cost-effective medium for enhancing the biomass of Clostridium butyricum HADIG-CB004,this study employed single-factor experiments and response surface methodology(RSM)to optimize the medium composition.Single-factor experiments results showed that glucose,sodium citrate,yeast extract,and fish peptone could significantly promote bacterial growth.RSM results showed that the optimal medium was composed of 13.77 g/L glucose,8.99 g/L yeast extract,11.05 g/L fish peptone,and 10.00 g/L sodium citrate,1.00 g/L ammonium sulfate,1.00 g/L L-cysteine hydrochloride,and 0.25 g/L manganese sulfate monohydrate,with initial pH value of 6.8.Validation experiments showed that under these conditions,the OD600 value reached 3.98,and the viable bacterial count reached 5×108 CFU/mL,which increased by 67.9%and 28.2%,respectively,compared with those under culture with the conventional reinforced Clostridium medium.The optimized C.butyricum proliferation medium with simple components and a low cost was suitable for the propagation of C.butyricum and preparation of postbiotics.