青藤碱-没食子酸纳米颗粒对脂多糖致小鼠急性肺损伤的保护作用OA

To explore effective and specific drugs for acute lung injury(ALI),sinomenine-gallic acid self-as-sembled nanoparticles(SGNPs)were prepared using one-step self-assembly method,and their protective effects and mechanisms against ALI were evaluated.The results showed that the average hydrodynamic diame-ter of SGNPs was(67.00±3.61)nm with a uniform spherical morphology.The hemolysis rate of SGNPs at a concentration of 0.8 mmol·L-1 was below the 5% safety threshold.,meeting the requirements for intrave-nous administration.SGNPs alleviated pathological damage in lung tissue,reduced inflammatory cell infiltra-tion,and significantly decreased the levels of serum IL-1β and IL-6,as well as the mRNA expression of IL-1β,IL-6,iNOS,and TNF-α in lung tissue.SGNPs could inhibit the phosphorylation levels of ERK,IκBα and NF-κB p65 in lung tissue,and SGNPs exhibited good biosafety both in vitro and in vivo.SGNPs exerted anti-inflammatory effects by regulating the MAPK/NF-κB signaling pathway,with simple preparation and excellent biocompatibility.