基于DNA重测序筛选加系西门塔尔牛超数排卵效果相关候选基因研究OA

Superovulation is an important step in the production of bovine embryos,but there are significant individual differences in the responses of donor cattle to superovulation treatment,and its genetic basis has not been fully clarified.This study took 60 16-month-old healthy Canadian Simmental donor cattle as the research subjects.Under the conditions of unified feeding management and superovulation program,high and low phenotypic populations were constructed based on the number of embryos and embryo availability.Blood samples were collected for whole-genome DNA resequencing.Through the combined selection elimi-nation analysis of population fixation index(FST)and genetic diversity ratio(Pi ratio),the candidate genes related to the effect of superovulation were systematically screened.The results indicated that a total of 10 candidate genes that might be involved in or regulate the process of superovulation were identified.Among them,BMPR1B,MMP 1,PGR,GHR,CD36 and ERBB4 were significantly correlated with the number of ovulation,while SOX5,CAMK1D,HDAC4 and BCL2 were significantly correlated with the availability of embryos.The above-mentioned genes are mainly involved in biological processes such as follicular deve-lopment,granulosa cell proliferation and oocyte maturation.The research results provide a molecular basis for analyzing the genetic basis of the superovulation trait in Simmental cattle and for precise screening of donor cattle.