黄芪甲苷和阿魏酸组分配伍调控糖脂代谢促糖尿病大鼠皮肤溃疡愈合的实验研究OA

Objective:To exploring the effect of astragaloside Ⅳ(AS-Ⅳ)and ferulic acid(FA)component compatibility on regulating glucose and lipid metabolism in diabetic skin ulcers and their impact on diabetic wound healing.Methods:A type 2 diabetic rat model was established.Successful model rats underwent a circular full-thickness skin excision with a diameter of 2 cm on the deep fascia on both sides of the spine to create a diabetic skin ulcer rat model(DCU).The rats were randomly divided into an AS-Ⅳ/FA group(DCU+50 mg/kg AS-Ⅳ/FA,mass ratio of AS-Ⅳ to FA 10:1)and a model group(DCU+0.9%NaCl),and a control group(0.9%NaCl)was was also established.All groups received intraperitoneal injections.Skin ulcer tissues were collected on days 7,14,and 21 to measure the wound healing rate.Western blotting was used to detect the expression of proteins related to the PI3K/Akt/GSK3β pathway and glucose and lipid metabolism(GCK,PFK-1,PKM,G6pase,LDLR,SREBP-1c,GLUT4,FOXO1).VEGF and eNOS levels were measured by enzyme linked immunosorbent assayc(ELISA).Results:Compared with the control group,the model group showed a lower wound healing rate(P<0.05),reduced expression levels of PI3K,Akt,GSK3β,GCK,PFK-1,PKM,G6pase,LDLR,GLUT4 and FOXO1,increased expression of SREBP-1c(P<0.05),and decreased expression of VEGF and eNOS(P<0.05).Compared with the model group,the AS-Ⅳ/FA group exhibited an increased wound healing rate(P<0.05),elevated expression levels of PI3K,Akt,GSK3β,GCK,PFK-1,PKM,G6pase,LDLR,GLUT4 and FOXO1,reduced expression of SREBP-1c(P<0.05),and increased expression of VEGF and eNOS(P<0.05).Conclusion:The combination of astragaloside IV and ferulic acid may promote diabetic wound healing by regulating glucose and lipid metabolism in diabetic skin ulcers,and this repair process may be related to the the activation of PI3K/Akt/GSK3β signaling pathway.