芜菁ABF基因家族成员鉴定及其在肉质根发育中的表达分析OA

ABF(ABRE binding factors)/AREB(ABA responsive element binding protein)transcription factors play crucial roles in plant growth,development,and abiotic stress responses.This study aimed to preliminarily investigate the functions of ABF genes during fleshy root development in turnip(Brassica rapa).Bioinformatics approaches were employed to identify and characterize members of ABF/AREB gene family in turnip,including analysis of protein physicochemical properties,gene structure and conserved motifs,chromosomal localization,promoter cis-acting elements,protein-protein interaction networks.Transcriptome data and quantitative real-time PCR(qRT-PCR)were used to analyze the expression patterns and levels of BcABF genes across different developmental stages of turnip fleshy roots,respectively.A total of 15 ABF/AREB family members were identified and classified into three subfamilies:Group A,Group B and Group C.These members were randomly distributed across eight chromosomes.Cis-acting element analysis revealed that BcABF promoters possess numerous elements related to growth and development,abiotic stress,and hormone response.Transcriptome analysis indicated that the expression levels of Group A members were significantly higher than those of Group B and C during fleshy root expansion.qRT-PCR analysis of six BcABF genes at different developmental stages of the fleshy roots of turnip revealed that BcABF2 gene was highly expressed specifically during the secondary root thickening stage(RTS),suggesting it may be a key regulator of turnip fleshy root expansion.These results provide a foundation for further functional characterization of key BcABF genes involved in promoting turnip fleshy root expansion and for variety improvement through molecular breeding.