施用有机物料对稻烟轮作土壤真菌群落的影响效应OA

[Objective]To investigate the ameliorative effects of organic materials on the soil micro-e-cology in rice-tobacco rotation systems.[Method]The experimental variety was the flue-cured tobacco Yunyan 87.Treatments included the application of chemical fertilizer alone[CK:base application of pure chemical fertilizer(1 050 kg/hm2)],chemical fertilizer combined with fermented cake fertilizer[T1:base application of pure chemical fertilizer(975 kg/hm2)+fermented cake fertilizer(450 kg/hm2)],rice straw incorporation[T2:pure chemical fertilizer as basal application(975 kg/hm2)+rice straw in-corporation(5 250 kg/hm2)],and a combination of the two[T3:pure chemical fertilizer as basal appli-cation(900 kg/hm2)+fermented cake fertilizer(450 kg/hm2)+straw incorporation(5 250 kg/hm2)].Soil samples were collected during the upper leaf maturation stage.The physicochemical properties of the soil,as well as differences in fungal community diversity and structural composition across the different treatments,were determined and analyzed using SPSS,R and OTU analysis.[Result]Compared with the control(CK)treatment,the application of organic materials did not significantly alter soil fungal alpha diversity but significantly altered community structure(P<0.05),with the T3 treatment having the stron-gest effect.The application of organic matter significantly reduced the relative abundance of the Basidio-mycota and Peniophoraceae,with these relative abundances showing a significant negative correlation with soil organic matter and available phosphorus contents;simultaneously,it significantly increased the relative abundance of the Hypocreaceae(P<0.05).Compared with the CK treatment,there were no sig-nificant differences in saprophytic,pathogenic and symbiotic microorganisms,whilst the relative abun-dance of plant pathogens in the organic matter application treatments was significantly reduced(P<0.05),with the lowest reduction of 33.90％observed in the T2 treatment and the greatest effect of 59.24％in the T3 treatment.Furthermore,the T3 treatment also increased the relative abundance of ec-tomycorrhizal fungi.[Conclusion]In rice-tobacco rotation fields,the application of chemical fertilizers combined with fermented cake fertilizer and rice straw return(T3)is an effective fertilization strategy for improving soil health in tobacco-growing field.