木槿SSR标记开发及杂种后代鉴定OA

[Objective]To support Hibiscus syriacus breeding and variety identification,this study de-veloped SSR markers based on transcriptome data and applied them for the molecular identification of hy-brid offspring.[Method]In this study,petals from different H.syriacus varieties were used as experi-mental materials.Transcriptome sequencing was performed using the Illumina platform,followed by anal-ysis of SSR loci using the MISA online tool.Specific primers were designed with Primer-BLAST online software.Total DNA was extracted from H.syriacus leaves using a kit method.PCR amplification was conducted using genomic DNA from 12 parent plants as templates,and the products were detected via agarose gel electrophoresis.Finally,polymorphic primers were screened.[Result]A total of 17 098 SSR sites were obtained,exhibiting diverse repeat motifs comprising 99 types.The number of motif types var-ied considerably among different categories:there were 2 types of mononucleotides,10 types of trinucle-otides,and 38 types of hexanucleotides.Sixty SSR loci with sequence lengths of 30 bp or more were se-lected.Three SSR markers with high polymorphism(HBG18,HBG7,and HBG21)were further devel-oped and successfully used to verify the authenticity of F1 progeny from five hybrid combinations:'Meiguimujin'×'Zhongguoxuefang','Lanniao'×'Hanbo','Fenhongxinchongban'×'Zhongguoxue-fang','Pufen'×'Hongxin','Lansexuefang'×'Changbaochongban'.[Conclusion]The SSR markers developed in this study exhibit high polymorphism and resolution,providing candidate markers for marker-assisted selection and promoting molecular-assisted breeding and germplasm conservation of Hibis-cus species.