白酒酒曲中淀粉酶产生菌筛选与产酶条件研究OA

Chinese fermentation starter harbors diverse microbial communities and rich enzyme systems that significantly influence liquor quality.Taking transparent circle diameter and amylase activity as evaluation indicators,this study isolated and screened superior amylase-producing strains from commercially available Chinese fermentation starter The effects of fermentation time,fermentation temperature,pH,carbon-nitrogen(C/N)ratio,and inoculation amount on results were systematically investigated,orthogonal experiments were conducted to optimize the enzyme production conditions.The results showed that the optimal conditions were:fermentation time of 96 h,fermentation temperature of 30℃,pH of 7.0,C/N ratio of 2∶5,and inoculum volume of 4%,and the amylase activity reached 90.6 U·mL-1.This study provides a theoretical basis for microbial screening from Chinese fermentation starter and fermentation production of related metabolites.