二甲双胍对卵母细胞成熟的作用机制研究OA

In vitro oocyte maturation(IVM)technology is a core tool in the field of animal assisted reproduction and biotechnology,and the quality of oocyte maturation directly affects fertilization rate and embryonic developmental competence.Oxidative stress,mitochondrial dysfunction,disrupted energy metabolism and endocrine abnormalities can impair both nuclear and cytoplasmic maturation of oocytes.Metformin,a widely used antidiabetic drug,not only improves insulin resistance and glucose and lipid metabolism but may also influence oocyte maturation by regulating AMPK signaling,improving mitochondrial function,reducing reactive oxygen species levels,modulating granulosa cell energy metabolism and alleviating endocrine disturbances associated with hyperandrogenism.However,the effects of metformin are not unidirectional and could be influenced by factors as species,dose,exposure duration,culture system and cellular metabolic status.This review summarizes the effects of metformin on the maturation quality and developmental competence of the oocytes,highlighting the experimental evidence in promoting and inhibitory effects,AMPK-related molecular mechanisms,granulosa cell-oocyte metabolic cooperation,and possible reasons for discrepant findings among studies.This paper intended to provide a theoretical basis for further research and practical application of metformin in animal reproduction and reproductive medicine.