木犀草素对动物卵巢氧化损伤的改善作用OA

[Objective]This study investigated the ameliorative effects of luteolin(LUT)on 3-nitropro-pionic acid(3-NPA)-induced ovarian oxidative stress injury,aiming to enhance its application poten-tial for improving reproductive performance in livestock production.[Method]An ovarian oxidative stress model was established by intraperitoneal injection of 3-NPA(20 mg·kg-1).Based on this model,forty 5-week-old female C57BL/6J mice were randomly assigned to a control group(CK),a model group(MG),and three luteolin treatment groups receiving low(25 mg·kg-1),medium(50 mg·kg-1),and high(100 mg·kg-1)doses,respectively.Luteolin was administered as a pretreatment for 3 weeks,and 3-NPA was injected during weeks 2 to 3 to induce ovarian oxidative stress injury.At the end of the experiment,growth performance,ovarian index,ovarian histomorphology,antioxidant parameters(T-AOC,SOD,CAT,GSH,and MDA),and serum hormone levels(AMH and E2)were measured.[Result]Compared with the CK group,the MG group showed a highly significant decrease in ovarian index(p<0.01),reduced numbers of mature follicles(p<0.01),increased numbers of atretic follicles(p<0.01),and a significant decrease in ovarian antioxidant capacity(p<0.05),indicating that 3-NPA successfully induced ovarian oxidative stress injury.In the dose screening experiment,compared with the MG group,luteolin treatment alleviated ovarian oxidative damage to varying degrees,with the medium-dose group(50 mg·kg-1)showing the most pronounced effect.Specifically,the ovarian index was markedly increased(p<0.001),the number of mature follicles was increased and the number of atretic follicles was markedly reduced(p<0.001),SOD activity and T-AOC levels were significantly elevated(p<0.01),and MDA molaity was significantly decreased(p<0.01).In addition,all luteolin-treated groups showed significantly increased serum AMH and E2 levels(p<0.05).[Conclu-sion]Luteolin pretreatment effectively alleviated 3-NPA-induced ovarian oxidative stress injury,improved ovarian reserve function and promoted follicular development,demonstrating potential appli-cation value of luteolin in alleviating ovarian oxidative damage and maintaining ovarian function.