枳实总黄酮的抗炎和抗氧化活性OA

Aurantii Fructus Immaturus has a long history of pharmacy use in China due to its rich biological activity.Ultrasonic-assisted extraction can destroy its cell wall via mechanical vibration,promote solvent penetration,and enhance bioactive component dissolution.In this study,five solvents of different polarities combined with ultrasonic-assisted technology were used to extract total flavonoids from Aurantii Fructus Immaturus.Antioxidant activity was evaluated by superoxide anion,DPPH and ABTS free radical scavenging assays,and ethanol was identified as the optimal extraction solvent.The anti-inflammatory activity results of ethanol extract on RAW264.7 cells indicated that the concentrations of 25,50,and 100 μg/mL of ethanol extract effectively alleviated the inflammation induced by lipopolysaccharide(LPS)in RAW264.7 cells by inhibiting the production of nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6).This study provides potential technical support for the application of ultrasonic-assisted extraction in the quality control of Aurantii Fructus Immaturus and lays an experimental foundation for its use in functional food and anti-inflammatory drug research and development.