鸡嘴簕的化学成分及生物活性研究OA

To study the chemical constituents and biological activities of Caesalpinia sinensis Hemsl.,separation and purification of its leaf and stem compounds were performed using separation techniques such as silica gel column chromatography,Sephadex LH-20 gel column chromatography,and HPLC.The purified compounds were characterized employing spectroscopic techniques including NMR/MS.A total of 11 compounds were isolated and identified from the leaves and stems and elucidated as neocaesalpin D(1),neocaesalpin N(2),neocaesalpin L(3),caesalpin F(4),7-oxodehydroabietic acid(5),3-deoxysappanone B(6),bonducellin(7),(3R)-dihydrobonducellin(8),(E)-7-methoxy-3-(4′-methoxybenzylidene)chroman-4-one(9),(aR,7R)-13-O-acetyl-7-dehydroxyprotosappanin B(10),and(aS,7R)-13-O-acetyl-7-dehydroxyprotosappanin B(11).All compounds were first isolated from this plant.The antiproliferative activity of cassane-type diterpenes(1-4)against the human breast cancer cell line MDA-MB-468 was tested,and the results demonstrated that compounds 1-4 exhibited no significant activity against the human breast cancer tumor cell line.