打箭菊萜类成分研究OA

In this study,systematic solvent extraction techniques and different chromatographic methods,such as normal-phase and reverse-phase silica gel column chromatography,along with semi-preparative high-performance liquid chromatography,were employed to isolate and purify the chemical components extracted from the ethyl acetate extract of Tanacetum tatsienense.The structures of the obtained compounds were determined using a range of spectroscopic techniques,including 1D and 2D NMR,high-resolution mass spectrometry,quantum chemical calculations,and conventional chemical methods.Six terpenoids were isolated and identified.Among them,compound 1 was recognized as a new norcarotenoid glycoside,named as tatsienenoside A(1).The remaining compounds were identified as known terpenoids,including 3β-hydroxy-β-ionone(2),1β-hydroxy-7-epi-costic acid methyl ester(3),arnidiol(4),faradiol(5),and maniladiol(6).The anti-proliferative activity of the isolated terpenoids was assessed using the CCK-8 method.The results indicated that arnidiol(4)exhibited moderate anti-proliferative activity against human non-small cell lung cancer A549 cells,with an IC50 value of(12.58±0.60)μmol/L.