濒危植物山豆根全长转录组测序与不同组织比较分析OA

Euchresta japonica is a rare and endangered legume species endemic to southern and southwestern China,valued for the anti-inflammatory and anti-cancer properties of its roots.Nevertheless,genomic information for this species remains largely unavailable.This study constructed a full-length and comparative transcriptome database to provide key molecular regulatory network and the synthesis mechanism of active compounds.Using PacBio SMRT third-generation sequencing technology,this study performed full-length transcriptome sequencing on a mixed sample from six tissues of E.japonica,combined with Illumina platform for various transcriptome profiling,thereby establishing the first high-quality transcriptomic resource for this species.The results were as follows:(1)A total of 21 703 high-quality Unigenes were obtained,of which 99.26％(21 543)were annotated in seven major gene functional databases.(2)A total of 1 543 transcription factors spanning 87 families were identified,and 7 649 SSR loci were detected distributed across 6 263 Unigenes,with compound SSR and tri-nucleotide repeats being the most abundant.(3)Comparative transcriptomic analysis across six tissues revealed 16 220 non-redundant differentially expressed genes(DEGs).The most pronounced differences were observed between fruit and inflorescence,and between lateral root and fruit,with substantial numbers of highly expressed genes detected in inflorescence,fruit,and lateral root.(4)Over half of the flavonoid biosynthesis-related genes were highly expressed in lateral root and/or inflorescence,consistent with metabolite accumulation patterns,indicating that these tissues are key sites for flavonoid biosynthesis.This study provides the first comprehensive transcriptome resource for the genus Euchresta,offering valuable genetic resources for elucidating the biosynthetic pathways of bioactive compounds,identifying key functional genes,and developing molecular markers.