水稻OsACS2及同源基因的结构特征和功能分析OA

To study the structural characteristics and functions of rice OsACS2 gene in rice development,the physicochemical properties,structure,and phylogenetic relationships of OsACS2 and its homologous proteins in rice,Arabidopsis thaliana,maize,wheat,barley,tomato and potato were analyzed using bioinformatics methods.Rice plants were treated with phytohormones abscisic acid(ABA)and methyl jasmonate(MeJA),and the expression levels of OsACS2 gene in rice roots and leaves were analyzed using real-time PCR.The results were as follows:(1)There were a total of 34 OsACS2 homologous genes found in rice,Arabidopsis thaliana,wheat,tomato,potato,maize and barley.(2)OsACS2 showed a closer relationship with its homologs in maize and wheat.(3)OsACS2 protein and its homologs showed similar subcellular localization,contained a conserved AMP(adenosine monophosphate)-binding domain,and had similar secondary and tertiary structures.(4)The promoter of the OsACS2 gene contained ABA and MeJA responsive elements,ABA treatment increased the expression of OsACS2 in roots and decreased its expression level in leaves,MeJA treatment resulted in a decrease in the expression level of OsACS2 in roots,but an increase in leaves.This study establishes a theoretical basis for further understanding the biological functions of OsACS2 and homologous genes.