人参皂苷Rg1对DSS诱导溃疡性结肠炎小鼠肠道屏障及菌群的调控作用OA

Objective:To investigate the regulatory effects and underlying mechanisms of Rg1on intestinal barrier function and gut mi-crobiota homeostasis in mice with DSS-induced UC.Methods:Male SPF-grade C57BL/6 mice were used to establish the DSS-induced UC model,and were randomly divided into the blank control group,model control group,and Rg1 low-dose[50 mg/(kg·d)],medium-dose[100 mg/(kg·d)]and high-dose[200 mg/(kg·d)]groups,with intragastric administration once daily for 14 consecutive days.The body weight,fecal traits and hematochezia of the mice were monitored,and the DAI was calculated.Colon length was measured,colonic pathological damage was evaluated by HE staining,the levels of inflammatory factors in colon tissues were detected by ELISA,and the intestinal microbiota in colonic contents were analyzed via 16S rDNA high-throughput sequencing.Results:Rg1 significantly alleviated DSS-induced body weight loss,re-duced DAI score,and increased colon length in mice(P<0.01).It also markedly down-regulated the levels of IL-1β,IL-6,TNF-α and MPO in colon tissues(P<0.01),attenuated colonic mucosal injury and inflammatory infiltration,increased goblet cell count,elevated intestinal micro-biota α-diversity,restored microbial community structure,corrected abnormal flora abundance at phylum and genus levels,and up-regulated the abundance of beneficial bacteria.Conclusions:Rg1 can effectively alleviate the symptoms and inflammatory response of DSS-induced UC in mice,and its mechanism is closely related to repairing the impaired intestinal barrier and regulating the dysregulated intestinal microbiota.This study provides experimental evidence for the clinical application of Rg1 in UC treatment.