基于网络药理学探求芦根-薏苡仁对药治疗胸外科术后肺炎的机制OA

Objective To systematically elucidate the mechanism of Rhizoma Phragmitis-Semen Coicis couplet medicines in preventing and treating postoperative pulmonary infection in thoracic surgery using Network Pharmacology combined with molecular docking technology.Methods Targets were screened using multiple databases including TCMSP and PubChem to obtain Venn diagrams.A Chinese medicine-component-target network was constructed,followed by GO and KEGG enrichment analysis and molecular docking verification.Results A total of 10 active components(e.g.,Stigmasterol,2-Monoolein,etc.)were screened,with 252 predicted targets,2 627 disease targets were obtained by integrating databases such as GeneCards,and 127 key drug-disease intersection targets were identified.A protein-protein interaction(PPI)network was constructed by STRING and subjected to topological analysis,identifying 10 core targets including MAPK3,JAK1,and PRKCD.GO and KEGG enrichment analyses revealed that the effects were mainly involved in G protein-coupled receptor signaling,calcium ion regulation,phospholipase D and sphingolipid signaling pathways,etc.Molecular docking results showed that the binding energies of core components to targets were all less than-5 kJ/mol,indicating stable binding.Conclusion Rhizoma Phragmitis-Semen Coicis couplet medicines exerts synergistic anti-inflammatory and immunomodulatory effects through a"multi-component,multi-target,multi-pathway"mode,providing a theoretical basis for the modern research and clinical transformation of this couplet medicines.