微小RNA-138在肝内胆管癌中的表达及生物功能研究OA

Objective To analyze the expression of microRNA-138(miR-138)in intrahepatic cholangiocarcinoma(ICC)and explore the effect of miR-138 on the biological function of ICC cells.Methods The ICC tissues and the adjacent tissues from 52 patients undergoing ICC surgery in Zhengzhou People's Hospital between June 2018 and June 2021 were retrospectively collected.The expression of miR-138 was detected by qRT-PCR,and the relationship between miR-138 and the pathological features and prognosis of ICC patients were analyzed.To culture human ICC cell line(HuCCT1 and RBE)and human normal intrahepatic bile duct cell line(HIBEC),and transfected with miR-138 mimics by liposome transfection technique.Cell proliferation,apoptosis,cycle,migration and invasion were detected by MTT,flow cytometry and Transwell methods.Western blotting was used to detect the expression of related proteins.Dual luciferase assay were used to analyze the targeting relationship between miR-138 and FOXC1.Results The expression level of miR-138 in ICC cancer tissues was significantly lower than that in adjacent tissues(P<0.05).MiR-138 was related to TNM stage,lymph node metastasis,vascular invasion and prognosis of ICC patients(P<0.05).The expression level of miR-138 in ICC cells(HuCCT1 and RBE)was significantly lower than that in HIBEC cells(all P<0.05).After up-regulation of miR-138,the proliferation,migration and invasion ability of ICC cells and the proportion of S phase cells were significantly reduced,and the apoptosis rate was significantly increased(P<0.05).Western blotting analysis showed that after up-regulation of miR-138,the expression levels of Bax and E-cadherin in ICC cells were significantly increased,while the expression levels of Bcl-2,CDK2,Cyclin D1,MMP-2,MMP-9 and Vimentin were significantly decreased(all P<0.05).Dual luciferase assay confirmed the targeting relationship between miR-138 and FOXC1.Conclusion The low expression of miR-138 in ICC tissues and cells is related to the occurrence,development and poor prognosis of ICC.The underlying mechanism may be related to the targeted regulation of FOXC1.