HPLC-MS/MS法测定血液中氯美扎酮OACHSSCD

Objective To establish a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for detecting chlormezanone in blood samples.Methods Acetonitrile was added to the samples to precipitate proteins.After vortex and ultrasonication,the mixture was centrifuged,and the supernatant was collected.It was then filtered using a 0.22 μm polytetrafluoroethylene mem-brane.Separation was performed using an InfinityLab Poroshell 120 EC-C18 column(100 mm×4.6 mm,2.7 μm).The mobile phase consisted of phase A(0.1%formic acid)and phase B(acetonitrile),with gra-dient elution at a flow rate of 0.4 mL/min.The mass spectrometer was operated with an electrospray ionization in positive ion mode and multiple reaction monitoring mode.Results Chlormezanone in blood samples showed good linearity within the tested range,with the correlation coefficients(r)all greater than 0.999.The limit of detection and the limit of quantitation of chlormezanone were 22.83 ng/mL and 100 ng/mL,respectively.The matrix effects were 2.1%-3.9%and recoveries were 88.8%-92.3%.Using this method,the mass concentration mass of chlormezanone detected in a positive sample was 1 217.23 ng/mL.Conclusion This method requires simple sample preparation and a small sample volume,and offers a wide linear range,making it suitable for the detection of chlormezanone in blood.