多孔调控的FeNC纳米酶用于四环素的比色检测OA

A colorimetric detection strategy for tetracycline(TC)based on a mesopore-modulated single-atom iron nanozyme is proposed.Compared to the unmodulated FeNC,the modulated nanozyme(FeNC-meso)features a concave porous nanostructure,which significantly promotes the exposure of active sites.Concurrently,FeNC-meso possesses more defects,which trigger partial charge transfer and facilitate the generation of reactive oxygen species(ROS),thereby markedly enhancing catalytic efficiency.Experimental results demonstrate that the specific activity(SA)and maximum reaction rate(vmax)of the FeNC-meso nanozyme are increased by factors of 2.01 and 2.06,respectively,compared to conventional FeNC.Leveraging its oxidase-like activity,a colorimetric detection system for TC is constructed.This system exhibits a linear detection range of 1.0 to 87.5 μmol/L and a limit of detection(LOD,S/N=3)of 0.23 μmol/L,demonstrating its effective capability for TC detection.The colorimetric detection platform exhibits excellent selectivity,anti-interference ability,and robust reliability in real sample analysis.The TC contents in real river and lake water samples are analyzed by this method,which are compared with those obtained by high-performance liquid chromatography(HPLC).The results from both methods are consistent.This study provides a promising research strategy for nanozyme structural regulation studies and antibiotic detection in the environment.