水相中稳定上转换荧光纳米探针的构建及其对硫醇分子的检测OA

Thiol compounds are key molecules for homeostatic regulation and environmental monitoring within bio-logical systems.The development of rapid and highly sensitive thiol detection methods holds significant importance for early disease diagnosis and environmental risk management.Fluorescent probes,owing to their high selectivity,high sensitivity,and non-invasive nature,have emerged as critical tools for detecting bio-small molecules,metal ions,and anions.This study employs IR806-sensitized upconversion nanoparticles(IR806-UCNP)as the core fluo-rescent probe system.By modifying the UCNP surface with polyethyleneimine(PEI),the probe's dispersibility and biocompatibility were effectively enhanced,conferring good hydrophilicity and stability in aqueous environments.Building upon this,the specific nucleophilic substitution reaction between thiol molecules and particular active groups in the IR806 dye was utilized to achieve specific fluorescence signal response of the probe to thiol molecules.This IR806-UCNP upconversion fluorescent probe,excited by near-infrared light,enables rapid and highly sensitive detection of thiol compounds.It exhibits a low detection limit of 0.1 μmol/L for glutathione(GSH),a typical thiol molecule in biological systems,providing a reliable method for subsequent applications in bioanalysis or environmen-tal monitoring.