α-烯醇化酶与鼻黏膜巨噬细胞胞葬缺陷的相关性及其对嗜酸性粒细胞型慢性鼻窦炎伴鼻息肉的鉴别价值OA

OBJECTIVE To determine the expression of α-enolase(ENO1)in chronic rhinosinusitis with nasal polyps(CRSwNP)and examine its relationships with macrophage efferocytosis and inflammation,and evaluate its diagnostic efficacy for distinguishing eosinophilic CRSwNP(ECRSwNP).METHODS We analyzed a public single-cell RNA-sequencing dataset to determine the expression level of ENO1 and predominant expressing cell types in CRSwNP,and validated ENO1 expression by qRT-PCR.Macrophage subsets related to efferocytosis were extracted and reclustered;differential expression of efferocytosis-related genes between ENO1-high and ENO1-low macrophages was assessed.Spearman correlation was used to examine associations between ENO1 expression and tissue inflammatory cell counts.Receiver operating characteristic(ROC)curves evaluated the ability of ENO1 to distinguish ECRSwNP.RESULTS ENO1 was most significantly elavated in ECRSwNP and prominently expressed in macrophages.Compared with ENO1 low-expressing macrophages,ENO1 high-expressing macrophages exhibited reduced expression of efferocytosis-related genes(CX3CR1,MERTK,GAS6).ENO1 showed a positive trend with tissue eosinophil counts(r=0.32,P=0.051).ROC analysis showed an area under the curve of 0.706(95%CI:0.542-0.870,P=0.028)for identifying ECRSwNP,with a sensitivity of 71.43%and a specificity of 66.67%.CONCLUSION ENO1 is upregulated in ECRSwNP,associates with impaired efferocytosis and inflammation,and shows moderate diagnostic value for distinguishing ECRSwNP.