长链非编码RNA MIR31HG在喉鳞状细胞癌中的表达及其与预后不良的关系OA
The expression of lncRNA MIR31HG in laryngeal squamous cell carcinoma and its relationship with poor prognosis
目的 探讨长链非编码RNA MIR31HG(long non-coding RNA MIR31HG,lncRNA MIR31HG)在喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)组织中的表达及其与预后不良的关系.方法 选取2022年1月～2024年12月中国人民解放军联勤保障部队第九八〇医院收治的113例LSCC患者,采集手术切除的喉癌组织及其癌旁组织,荧光定量PCR技术检测lncRNA MIR31HG表达水平.根据lncRNA MIR31HG诊断LSCC的最佳截值,将LSCC患者分为高表达组(lncRNA MIR31HG≥1.61)和低表达组(lncRNA MIR31HG<1.61),比较不同lncRNA MIR31HG表达与LSCC患者临床病理特征的差异.术后随访并记录LSCC患者的总生存期(OS)和无进展生存期(PFS),使用Kaplan-Meier法绘制生存曲线.应用单因素及多因素Cox回归分析影响LSCC患者预后不良的独立危险因素.结果 lncRNA MIR31HG在LSCC组织表达水平明显高于癌旁组织(2.17±0.58 vs.0.81±0.16,t=16.573,P<0.001).受试者工作特征(ROC)曲线结果显示,lncRNA MIR31HG诊断LSCC的最佳截值为1.61,AUC为0.894(95%CI=0.837～0.928).Ⅲ～Ⅳ期(90.0%vs.10.0%)、T3～T4级(87.8%vs.12.2%)、N2～N3级(96.9%vs.3.1%)、M1级(94.4%vs.5.6%)、低分化(92.3%vs.7.7%)及淋巴转移(90.5%vs.9.5%)在lncRNA MIR31HG高表达组的发生率明显高于低表达组(P<0.05).Kaplan-Meier生存曲线结果分析显示,高表达组OS(70.84%vs.90.15%)及PFS(52.36%vs.74.18%)均明显低于低表达组(P<0.05).单因素及多因素Cox回归分析显示,Ⅲ～Ⅳ期、T3～T4级、N2～N3级、M1级、低分化、有淋巴结转移及lncRNA MIR31HG高表达是LSCC患者预后不良的危险因素(P<0.05).结论 lncRNA MIR31HG在LSCC组织中呈高表达,其高表达与肿瘤分期、低分化及淋巴结转移密切相关,是影响LSCC患者预后不良的危险因素.
OBJECTIVE To investigate the expression of long noncoding RNA MIR31HG(lncRNA MIR31HG)in laryngeal squamous cell carcinoma(LSCC)tissues and its relationship with poor prognosis.METHODS A total of 113 LSCC patients admitted to the 980th Hospital of Joint Logistics Support Force of the Chinese People's Liberation Army from January 2022 to December 2024 were selected.Tumor tissues and adjacent normal tissues were collected during surgical resection,and the expression level of lncRNA MIR31HG was detected by quantitative real-time PCR(qPCR).According to the optimal cut-off value of lncRNA MIR31HG for diagnosing LSCC,LSCC patients were divided into the high expression group(lncRNA MIR31HG≥1.61)and the low expression group(lncRNA MIR31HG<1.61).Compare the differences in the expression of different lncRNA MIR31HG and the clinicopathological characteristics of patients with LSCC.Postoperative follow-up was conducted to record the overall survival(OS)and progression-free survival(PFS)of LSCC patients,and Kaplan-Meier curves were generated to illustrate survival outcomes.Univariate and multivariate Cox regression analyses were performed to identify independent risk factors associated with poor prognosis in LSCC patients.RESULTS The expression level of lncRNA MIR31HG in LSCC tissues(2.17±0.58 vs.0.81±0.16)was significantly higher than that in adjacent tissues(t=16.573,P<0.001).The ROC curve showed that the optimal cut-off value of lncRNA MIR31HG for diagnosing LSCC was 1.61,and the AUC was 0.894(95%CI=0.837-0.928).The incidences of stage Ⅲ-Ⅳ(90.0%vs.10.0%),T3-T4(87.8%vs.12.2%),N2-N3(96.9%vs.3.1%),M1(94.4%vs.5.6%),poor differentiation(92.3%vs.7.7%),and lymph node metastasis(90.5%vs.9.5%)were significantly higher in the lncRNA MIR31HG high-expression group than in the low-expression group(all P<0.05).Kaplan-Meier survival analysis revealed that the high-expression group exhibited significantly lower OS(70.84%vs.90.15%)and PFS(52.36%vs.74.18%)compared with the low-expression group(P<0.05).Univariate and multivariate Cox regression analyses indicated that stage III-IV disease,T3-T4 stage,N2-N3 stage,M1 stage,poor differentiation,lymph node metastasis and high expression of lncRNA MIR31HG were risk factors for poor prognosis in LSCC patients(P<0.05).CONCLUSION lncRNA MIR31HG is highly expressed in LSCC tissues,and its elevated expression is closely correlated with advanced tumor stage,poor differentiation,and lymph node metastasis,serving as an independent risk factor for unfavorable prognosis in LSCC patients.
孔延男;刘亮;牛云峰;赵惠玲
中国人民解放军联勤保障部队第九八〇医院病理科,河北 石家庄 050000中国人民解放军联勤保障部队第九八〇医院病理科,河北 石家庄 050000中国人民解放军联勤保障部队第九八〇医院病理科,河北 石家庄 050000中国人民解放军联勤保障部队第九八〇医院病理科,河北 石家庄 050000
喉肿瘤癌,鳞状细胞癌预后长链非编码RNA MIR31HG临床病理
Laryngeal NeoplasmsCarcinoma,Squamous CellPrognosislncRNA MIR31HGclinical pathology
《中国耳鼻咽喉头颈外科》 2026 (4)
181-185,5
河北省医学科学研究课题(20240854)
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