具有可注射性及抗菌性能的动态交联水凝胶OA

The clinical management of chronic wounds is frequently challenged by bacterial infection and biofilm formation.To address this,we developed an injectable hydrogel,denoted as AMCS@ODex,via dynamic Schiff base bonding between amide-modified chitosan(AMCS)and oxidized dextran(ODex).This crosslinking strategy not only mitigated the inherent limitations of chitosan,such as poor solubility and mechanical properties,but also endowed the hydrogel with excellent shear-thinning behaviors.AMCS@ODex exhibited antibacterial efficacy against Escherichia coli(E.coli)and methicillin-resistant Staphylococcus aureus(MRSA),and the antibacterial rates against E.coli and MRSA were 58%and 73%,respectively.Furthermore,it effectively disrupted pre-formed MRSA biofilms,leading to a disruption rate approximately 50%.Critically,the hydrogel exhibited outstanding biocompatibility,supporting over 90%viability of L929 fibroblast cells.By integrating good antibacterial performance,with desirable mechanical properties and high biocompatibility,the AMCS@ODex hydrogel presents potential biomedical applications,particularly in the fight against bacterial infections.