抗原与STING激动剂共价连接的响应释放型纳米疫苗促进树突状细胞成熟OA

To develop a nanovaccine capable of synchronously delivering antigens and adjuvants for effective dendritic cell(DC)maturation,we covalently conjugated the stimulator of interferon gene(STING)agonist MSA-2 with the model antigen CSIINFEKL via a cleavable linker.The resulting prodrug was co-assembled with phosphoethanolamine-polyethylene glycol(DSPE-PEG)to form a nanovaccine(termed NVAA).The NVAA exhibited a uniform spherical morphology with an average hydrodynamic diameter of approximately 20 nm and a zeta potential of-14 mV.Upon internalization by DCs,the covalently conjugated antigen and STING agonist enabled their synchronized intracellular delivery,leading to efficient activation of the STING signaling pathway.As a result,NVAA significantly enhanced the secretion of IFN-β,IL-6,and IL-12,and upregulated the expression of costimulatory molecules CD80 and CD86 on DCs.These findings demonstrate that the covalent conjugation-based nanovaccine NVAA effectively promotes DC maturation via STING pathway activation,providing a promising strategy for cancer immunotherapy.