赤霉素协同发芽基质促进紫云英陈种萌发及幼苗生长OA

The low germination rate of long-term stored seed of Chinese milk vetch(Astragalus sinicus L.)is an important factor limiting production,and exploring methods to promote the germination can improve their uti-lization and productivity.In this study,Chinese milk vetch seeds stored for 17 years and 3 years were used as materials.After soaking in distilled water(CK)and 400 mg·L-1 gibberellin(GA3(400))solution,the seeds were placed in different germination substrates,including paper bed(FZ),coconut coir(YK),and vermiculite(ZS),and the germination rate and seedling growth were measured.GA3,germination substrate and the inter-action between the two treatments significantly promoted the germination and seedling growth of Chinese milk vetch.Compared with CK,after soaking in GA3,the germination rate of seeds stored for 17 years and 3 years was increased by 1.5 fold and 0.9 fold,the plant height increased by 42.6%and 72.5%,the primary root length increased by 19.1%and 45.1%,the fresh weight of aboveground increased by 12.4%and 32.3%,the fresh weight of roots increased by 7.8%and 62.8%,respectively.After soaking in GA3,for seeds stored for 17 years and 3 years,the plant height,main root length,and root fresh weight was significantly higher under ZS than under FZ and YK.Using the membership function method to comprehensively evaluate each treat-ment,soaking seeds in GA3 and planting in ZS was a suitable method to promote seed germination and seedling growth of long-term stored seed of Chinese milk vetch.