基于网络药理学探讨五味消毒饮治疗盆腔炎的作用机制OA

Objective To employ the network pharmacology approach and in vitro cell experiments to investigate the thera-peutic effects and molecular mechanisms of Wuwei Xiaodu Decoction in the treatment of pelvic inflammatory disease in mice.Methods Firstly,a mouse model of pelvic inflammatory disease was constructed,and the therapeutic effect of Wuwei Xiaodu Decoction on pelvic inflammatory disease mice was evaluated through weight changes,morphological examination of pelvic tis-sues,and pathological observation of uterine tissues as evaluation indicators;TCMSP and Pubchem databases were used to screen out the active ingredients and targets of Wuwei Xiaodu Decoction,and the STRING platform was used to construct a protein-pro-tein interaction(PPI)network;the Metascape database was used to perform GO and KEGG enrichment analysis on core targets;the Cytoscape software was used to construct a component disease target map;the LPS was used to induce RAW264.7 inflamma-tory cell model,and the effect of Wuwei Xiaodu Decoction on the secretion of nitric oxide(NO)in cells was detected by Griess method;the Western blot was used to detect the effects of Wuwei Xiaodu Decoction extract on iNOS,p-NF-κB、NF-κB,p-JNK、JNK,p-ERK and ERK protein levels in RAW264.7 cells.Results In vivo,Wuwei Xiaodu Decoction could be used to improve uterine congestion,swelling,and adhesion to surrounding tissues in the mouse pelvic inflammatory disease model.Through net-work pharmacology screening,there were 49 potential active ingredients and 544 intersecting targets in Wuwei Xiaodu Decoction for treating pelvic inflammatory disease.Protein interaction prediction suggested that caspase-3,STAT3,and SRC might be key targets for Wuwei Xiaodu Decoction in treating pelvic inflammatory disease,mainly involving the glucocorticoid receptor regulato-ry pathway and hypoxia inducible factor-1 signaling pathway.In vitro,Wuwei Xiaodu Decoction significantly reduced the secre-tion of NO in RAW264.7 cells induced by LPS.Wuwei Xiaodu Decoction could inhibit the expression of iNOS protein and the activation of key proteins in the NF-κB and MAPK signaling pathways induced by LPS in RAW264.7 cells.Conclusion Wuwei Xiaodu Decoction has a relieving effect on pelvic inflammatory disease in mice.Based on network pharmacology research,Wuwei Xiaodu Decoction has the characteristics of multi-target and multi pathway synergistic effects to exert its therapeutic effects.Cell experiments show that Wuwei Xiaodu Decoction inhibits abnormal pathway activation of NF-κB and MAPK signaling pathways.