基于两样本孟德尔随机化探究干果摄入与2型糖尿病的因果关系OA

Objective To explore the causal association between nut intake and the risk of type 2 diabetes mellitus with two-sample Mendelian randomization(MR).Methods Genome-wide association study(GWAS)-based pooled data were used to perform two-sample Mendelian randomization with nut intake as the exposure and type 2 diabetes mellitus as the outcome.The primary analysis method was inverse variance weighting(IVW).F-value statistics and Cochran's Q statistics were used to test the stability and heterogeneity of instrumental variables.MR-Egger regression,weighted median method,simple plurality-based estimation,weighted plurality-based estimation,and leave-one-out method were used for sensitivity analyses.Results A total of 41 single-nucleotide polymorphism loci were included as instrumental variables for nut intake.The results of Mendelian randomi-zation indicated an inverse causal association between nut intake and type 2 diabetes mellitus(IVW method OR:0.48,95％confidence interval:0.30-0.76,P=0.002),and there was no pleiotropy.The results obtained from the weighted median and leave-one-out methods were consistent with IVW.Conclusion There is a negative causal relationship between nut intake and the onset of type 2 diabetes mellitus.