沉默RND1对舌鳞状细胞癌增殖、凋亡、侵袭和迁移的影响OA

Objective To evaluate the effects of RND1 silencing on the proliferation,apoptosis,invasion,and migration of tongue squamous cell carcinoma(TSCC)cells,and to explore the functional role of RND1 in TSCC progression.Methods RND1 protein expression levels in TSCC tissues and paired adjacent non-cancerous tissues were detected by Western blot and im-munohistochemistry.RND1 expression was silenced with siRNA in TSCC cell lines.Cell proliferation was assessed with the Cell Counting Kit-8(CCK-8)assay,apoptosis was evaluated with flow cytometry,and cell invasion and migration were assessed with Transwell and wound healing assays.In addition,Western blot analysis was performed to examine the expression of epithelial-mesenchymal transition(EMT)-related proteins and downstream signaling pathway-associated proteins.Results RND1 protein expression was significantly higher in TSCC tissues than in adjacent normal tissues(P＜0.05).Compared with the blank and negative control groups,RND1-silenced cells exhibited significantly reduced proliferation,increased apoptosis,and markedly de-creased invasion and migration,with all differences being statistically significant(P＜0.05 or＜0.01).Meanwhile,the expres-sion of the epithelial marker E-cadherin was upregulated,whereas the expression of the mesenchymal markers N-cadherin and Vimentin,as well as downstream signaling pathway-related proteins,was downregulated(P＜0.05).Conclusion RND1 plays a tumor-promoting role in TSCC.Silencing RND1 attenuates cell proliferation,invasion,and migration,and promotes apoptosis by suppressing the EMT process,suggesting that RND1 may serve as a potential therapeutic target for TSCC.