血清中lncRNA H19和miR-22-3p对急性心肌梗死患者的诊断价值OA

Objective To explore the expression levels of long non-coding RNA(lncRNA)H19 and microRNA-22-3p(miR-22-3p)in the serum of patients with acute myocardial infarction(AMI)and their diagnostic value for AMI.Methods A total of 176 AMI patients were selected as the experimental group and were divided into ST-segment elevation myocardial infarction(STEMI)group(n=95)and non-ST-segment elevation myocardial infarc-tion(NSTEMI)group(n=81)based on their medical history and electrocardiogram.Meanwhile,156 patients with negative angiography during the same period were selected as the control group(CON group).The relative expres-sion levels of lncRNA H19 and miR-22-3p in the serum of the two groups were detected by real-time fluorescence quantitative polymerase chain reaction.The diagnostic value of miR-22-3p and lncRNA H19 in AMI was evaluated by receiver operating characteristic curve(ROC)analysis.The levels of serum cardiac troponin I(cTnI),creatine kinase(CK),creatine kinase MB isoenzyme(CK-MB),and high-sensitivity C-reactive protein(CRP)were de-tected in all study subjects.Spearman correlation analyses were used to evaluate the correlations between lncRNA H19 and miR-22-3p and cTnI,CK,CK-MB and CRP.Results Compared with the control group,the expression level of lncRNA H19 was upregulated and the expression level of miR-22-3p was downregulated in the AMI group(all P<0.01);compared with the NSTEMI group,the expression level of lncRNA H19 was upregulated and the ex-pression level of miR-22-3p was downregulated in the STEMI group(P<0.01).The areas under the ROC curves(AUC)for the diagnosis of AMI by miR-22-3p,lncRNA H19 and their combination were 0.555,0.977,and 0.983,respectively.lncRNA H19 was positively correlated with cTnI,CK,CK-MB and CRP(P<0.05),and negatively correlated with left ventricular ejection fraction(LVEF)(P<0.001);miR-22-3p was negatively corre-lated with cTnI,CK and CRP,and positively correlated with LVEF(P<0.05).Conclusion The expression level of lncRNA H19 is elevated and the expression level of miR-22-3p decreased in AMI patients,and their levels may have potential value as an auxiliary biomarker for AMI diagnosis and cardiac function assessment.