多效唑对大豆籽粒蛋白质含量的影响及转录组分析OA

To identify the key genes regulating soybean seed protein content in response to paclobutrazol,Baodou 1519 was used as the material in this study.A control group(CK)was sprayed with clean water.During the flowering and podding stage,a 1 000-fold diluted solution of 15%paclobutrazol wettable powder was sprayed every 7 days.3 treatment groups were established:T1(sprayed once),T2(sprayed twice),and T3(sprayed three times).The seed protein content of each treatment was analyzed,and fresh seeds were collected at the filling stage for transcriptomic analysis.The results showed that treatment T2 exhibited the highest crude protein content(46.11%)and water-soluble protein content(39.42%).Good sequencing quality was obtained for all treatment samples,with the proportion of valid reads exceeding 97.31%(average of 97.68%).Among the detected genes,1 109 genes were expressed exclusively in T1,6 495 genes exclusively in T2,and 636 genes exclusively in T3.A total of 8 451 differentially expressed genes(DEGs)were identified between the T2 and CK groups.GO and KEGG enrichment analyses indicated that the DEGs between T2 and CK were mainly enriched in biological processes such as response to oxygen-containing compounds,negative regulation of hydrolase activity,response to abiotic stimuli,and response to lipids.KEGG pathway analysis showed that the DEGs were primarily enriched in starch and sucrose metabolism,fatty acid metabolism,and photosynthesis metabolism.Further analysis revealed that 56 transcription factors were differentially expressed,and the expression levels of transcription factors such as NAC,ERF,C2H2,and MYB were found to be up-regulated in response to paclobutrazol stimulation.10 up-regulated DEGs(including GLYMA_20G029600)and 10 down-regulated DEGs(including GLYMA_16G084000)were identified as potentially involved in the regulation of soybean seed protein content by paclobutrazol.This study provides a reference for the rational application of paclobutrazol.