铜绿假单胞菌注射液对健康供者免疫功能的影响OA

Objective:To investigate impact of Pseudomonas aeruginosa-mannose sensitive hemagglutination(PA-MSHA)on immune status of healthy donors and to provide a theoretical foundation for enhancing immunity in sub-healthy individuals.Methods:A total of 15 healthy donors were recruited from the First Affiliated Hospital of Zhengzhou University and received subcutaneous injec-tions of PA-MSHA.Peripheral blood samples were collected prior to injection and 30 days post-injection,flow cytometric analysis was used to assess proportions of immune cell subsets,expressions of T cell surface activation and inhibition markers,as well as capacity of T cells and NK cells to secrete pro-inflammatory cytokines.High-throughput sequencing was employed to analyze T cell receptor(TCR)diversity.Results:Following PA-MSHA administration,there was a notable increase in CD4/CD8 among participants.Concur-rently,an elevation in activating marker expressions was observed alongside a reduction in inhibitory marker expressions on T cells.Furthermore,both T cells and NK cells exhibited significantly enhanced secretion capabilities for pro-inflammatory cytokines such as IFN-γ,Granzyme B and Perforin;additionally,an increase in peripheral blood TCR diversity was noted.In vitro experiments demon-strated that CD3+T cells treated with PA-MSHA displayed markedly improved functionality compared to control groups.Conclusion:PA-MSHA substantially enhances immune status of healthy donors and offers a viable therapeutic approach for bolstering immunity in sub-healthy populations.