双源生物炭耦合复合菌剂协同预处理玉米秸秆研究OA

To enhance the anaerobic digestion efficiency of corn stalks,a dual-source biochar coupled with a composite mi-crobial agent was developed for a synergistic pretreatment system.Through single-factor experiments,the effects of biochar addition(5％～15％)and the ratio of dual-source biochar(corn stalk biochar∶hemp stalk biochar=1∶0,1∶1,1∶2,2∶1,0∶1)on the degradation of lignocellulose,the activity of key enzymes,and the generation of reducing sugars during the pretreatment process were systematically studied.The results indicated that the addition of dual-source biochar could signif-icantly improve the pretreatment efficiency of the composite microbial agent(Trichoderma reesei∶Trichoderma viride∶Phan-erochaete chrysosporium=1∶1∶2).The optimal parameters were a biochar addition of 12.5％(based on the dry weight of the stalks)and a dual-source biochar ratio of 1∶1.Under these conditions,after 15 days of pretreatment,the degradation rates of cellulose,hemicellulose and lignin reached 43.31％,41.05％and 25.82％respectively.Compared with the blank control group CK,they increased by 200.0％,216.8％and 370.3％respectively.Compared with the single com-pound bacteria group M,they increased by 70.1％,101.5％and 161.2％respectively.The peak activities of cellulase,xylanase,and laccase reached 158.7 U·mnL-1,142.2 U·mL-1,and 118.7 U·mnL-1,respectively,and the stability of en-zyme activity was enhanced.The peak accumulation of reducing sugars reached 16.8 mg·g-1.Structural characterization(SEM,FTIR,XRD)indicated that the synergistic pretreatment effectively disrupted the dense structure of the stalks,and the crystallinity of cellulose decreased from 35.2％to 22.6％.Biochar,through multiple mechanisms such as adsorbing microorganisms and enzymes,regulating the microenvironment of the system,and serving as an electron shuttle,produced a synergistic effect with the composite microbial agent,providing high-quality substrates for subsequent anaerobic digestion and offering technical references for the efficient energy utilization of corn stalks.