亚麻bHLH转录因子基因家族鉴定与表达分析OA

The basic Helix-Loop-Helix(bHLH)is a class of transcription factors that are involved in the regulation of plant growth and development,morphogenesis,and response to environmental stress.Based on the flax(Linum usitatissimum L.)genome database,this study used bioinformatics methods to analyze the chromosome localization,physicochemical properties,conserved motifs,conserved domains,gene structure,promoter cis-acting elements,phylogeretic relationships,and expression patterns in the middle stem at different developmental stages of the bHLH transcription factor family.The results showed that there were 159 members of the flax bHLH gene family,which were unevenly distributed among 15 chromosomes.Gene conservation analysis indicated that Motif 1 and Motif 2(bHLH superfamily)were the conserved protein sequences in the flax bHLH family.The prediction of promoter cis-acting elements revealed that the flax bHLH gene family played important roles in light response,plant hormones,stress responses,growth and development,and secondary metabolism synthesis.Evolutionary analysis showed that the 159 members of flax were divided into 16 sub-families.The collinearity analysis with Arabidopsis thaliana,Gossypium hirsutum,and Populus trichocarpa revealed that flax had the most collinear genes with G.hirsutum,suggesting a possible common ancestor.RNA-seq analysis indicated that the bHLH family members were expressed at different developmental stages in the middle part of the flax stem,and the LusbHLH101gene had the highest expression level.