干旱促进ABA积累以诱导青稞HVA1基因表达OA

Using hulless barley cultivar Kunlun 12 as experimental material,PEG 6000 was used to simulate drought stress,and treatments with abscisic acid(ABA)and the ABA biosynthesis inhibitor fluridone were employed to analyze the relationship between HVA1 gene expression and drought and ABA.The results showed that as the concentration of PEG 6000 pretreatment increased,leaf relative moisture content decreased,relative electrical conductivity decreased first and then increased,and ABA content and HVA1 gene relative expression increased first and then decreased.As the duration of 15%PEG 6000 pretreatment increased,leaf relative moisture content decreased,relative electrical conductivity increased,and ABA content and HVA1 gene relative expression first increased and then decreased.Compared with 15%PEG 6000 pretreatment alone,the combination of 15%PEG 6000 and fluridone significantly decreased endogenous ABA content and HVA1 gene relative expression,but the decrease of HVA1 gene relative expression was greater than that of ABA content.The HVA1 gene relative expression under the combination of ABA pretreatment and 15%PEG 6000 treatment was significantly lower than that of either treatment alone,showing no additive effect.In conclusion,leaf relative moisture content and electrical conductivity can be used to effectively evaluate the drought response of hulless barley.Drought stress promotes ABA accumulation,thereby inducing HVA1 gene expression.HVA1 gene expression under drought stress is regulated by other pathways in addition to endogenous ABA.