一株太子参植物内生真菌链格孢菌次生代谢产物的研究OA

Sixteen compounds were isolated from the solid rice culture of the endophytic fungus Alternaria alstroemeriae WZ-419 derived from Pseudostellariae Radix,using silica gel,ODS,Sephadex LH-20 column chromatography,and preparative HPLC.Their structures were elucidated based on physicochemical properties and comprehensive spectroscopic analyses,and identified as alterpenoid A(1),tricycloalternarene A(2),altenusin(3),4'-epialtenuene(4),altenuene(5),alternariol(6),altenuisol(7),3-hydroxyalternariol-5-O-methylether(8),alternariol-9-methyl ether(9),alternariol 1'-hydroxy-9-methyl ether(10),stemphyperylenol(11),dihydroalterperylenol(12),alterinone A(13),4-hydroxy-3-methoxy-5-(2E,6E-3,7,11-trimethyldodeca-2,6,10-trien-1-yl)benzoic acid(14),hexylitaconic acid(15),and altechromone A(16).Among them,compound 1 and 13 were new,and compounds 14 and 15 were isolated from the genus Alternaria for the first time.The antibacterial a ctivities of compounds 1-16 were evaluated in vitro.Compounds 3,6-8,10,11,13,and 14 exhibited notable inhibitory effects against Gram-positive bacteria,with minimal inhibitory concentrations ranging from 8 to 64 µg/mL.This study isolated a series of antimicrobial metabolites from the endophytic fungi of Pseudostellariae Radix,providing new insights into their bioactive constituents and laying a foundation for further pharmacological investigations.